Home Instruments & Equipments Multi-Modal Imaging and Measurements (I3M) unit Laser Capture Microdissection (LCM) Microscope

Laser Capture Microdissection (LCM) Microscope

An optical microscope equipped with a focused laser beam to cut and isolate regions of interest at the micrometer scale.

The laser microdissection microscope allows the collection of micro-samples for cell-scale transcriptomic analyses. The PALM Microbeam uses a concentrated laser to cut and isolate the target sample at micrometer precision. A laser “catapult” then ejects the selected region quickly and contamination-free. Captured RNA can subsequently be extracted using specific protocols and analysed via quantitative PCR or RNA sequencing (RNAseq). Other potential applications include metabolomics, proteomics, microbial isolation, and single-cell genomics, although these have not yet been developed in the laboratory.

Applications:

  • Transcriptomic studies of tissues infected by pathogens
  • Analysis of mycorrhizal symbiosis
  • Investigation of cell-scale physiological processes
  • Studies of wood tissue
     

 

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See also

  • https://www.youtube.com/watch?v=Qs8onaLfq8c
  • Durand M, Cohen D, Aubry N, Buré C, Tomášková I, Hummel I, Brendel O, Le Thiec D. 2020. Element content and expression of genes of interest in guard cells are connected to spatiotemporal variations in stomatal conductance. Plant Cell Environ. 43: 87–102. doi : 10.1111/pce.13644
  • Heinen RB, Bienert GP, Cohen D, Chevalier AS, Uehlein N, Hachez C, Kaldenhoff R, Le Thiec D, Chaumont F. 2014. Expression and characterization of plasma membrane aquaporins in stomatal complexes of Zea mays. Plant Mol Biol. 86(3):335-50. doi: 10.1007/s11103-014-0232-7
  • Dumont J, Cohen D, Gérard J, Jolivet Y, Dizengremel P, LE Thiec D. 2014. Distinct responses to ozone of abaxial and adaxial stomata in three Euramerican poplar genotypes. Plant Cell Environ. 37(9):2064-76. doi: 10.1111/pce.12293

By: CM Publication date: 19 January 2022 Modification date: 01 September 2025