Zeiss LSM 780 Laser Scanning Confocal Microscope

An optical microscope enabling 3D imaging of biological samples or materials using reflected light or fluorescence. The available model is equipped with laser lines at 405 nm, 458 nm, 488 nm, 514 nm, 561 nm, and 633 nm, and can acquire images between 405 nm and 700 nm using various detectors, including a highly sensitive spectral detector (GaAsP). It allows emission spectrum acquisition and spectral unmixing. Equipped with long-working-distance objectives 10X/0.3, 20X/0.8, 50X/0.95, and immersion objectives 40X/1.2 and 63X/1.4. An incubation chamber enables temperature and CO₂ concentration control for in vivo imaging. Special holders are available for performing correlative confocal/SEM microscopy on the platform.

Main Imaging Applications

• Cellular-scale imaging of plant and fungal tissues
• Observation of plant/fungus/bacteria interactions
• Bacterial biofilm studies
• Protein localization
• Protein–protein interaction analysis
   

Main Techniques Used

• Staining with fluorochromes (DAPI, propidium iodide, WGA, TOPRO, etc.)
• Fluorescence in situ hybridization (FISH)
• Protein or microorganism/plant labeling (GFP, mCherry, YFP, etc.)
• Immunolabelling
   

Contact:

• Equipment coordinators: Aurélie Deveau aurelie.deveau@inrae.fr - Frédéric Guinet frederic.guinet@univ-lorraine.fr - Christophe Rose christophe.rose@inrae.fr 
• Unit coordinator : Julien Ruelle julien.ruelle@inrae.fr