Cryo-Microscopy-MEB

Objectives

• Discover and handle various tools and techniques for preparing frozen biological samples and Cryo-microscopy.
• Prepare and observe biological (plant) samples under the electron beam of a FEG-SEM equipped with Cryo-microscopy or with a cooled stage (observations in controlled pressure mode with a Peltier-effect stage).
   

Target Audience

Priority is given to members of the INRAE Microscopists Network (RµI) and INRAE staff involved in Cryo-SEM imaging.
Prerequisites: Engineers, technicians, or researchers with good experience or knowledge of scanning electron microscopy (SEM) tools.

Instructors

• Christophe Rose – SILVATECH Engineer – Head of the Imaging & Microscopy Unit
• Nicolas Angeli – Head of the SILVATECH Platform
   

Program

• Theoretical Input:
  Freezing of water and hydrated samples
  Techniques and equipment in Cryo-microscopy
  Handling of liquid nitrogen and cold objects
• Practical Workshops:
  Observation in controlled pressure mode with cooled stage
  Cryo-sample preparation and observations under SEM electron beam
  Rapid freezing, fracture, vacuum sublimation, cold metallization
  High-resolution observations, micro-analyses (if possible)
   

Equipment

• Peltier-effect cooled stage (-50°C / +50°C)
• ACE 600 Cryo-coater
• Cryo-stage + VCT 100 cryo-transfer module
• Sigma HD VP FEG-SEM + EDS spectrometer
   

Practical Information

• Location:
  INRAE Grand Est – Nancy Center
  UMR 1134 SILVATECH
  54280 Champenoux
  FRANCE
• Training Cost: €500 (excluding travel and accommodation expenses)
  For RµI network members or INRA staff: 50% covered by FTLV (€250 charged to the agent's unit)
  For other EPST organizations: Please contact us
• Lunch costs covered by the Nancy Training Service
• Duration: 2.5 days – Maximum 4 participants
   

CONTACT

• Corinne Weigerding –FTLV GEN : corinne.weigerding@inra.fr - 03.83.39.73.31
• Christophe Rose – SILVATECH : christophe.rose@inra.fr - 03.83.39.41.22